Potential Problems with the Reliability of Pcr Based Diagnostic Methods Related to Plant Viruses Sequence Variation
نویسنده
چکیده
Selected problems related to non-uniformity of plant virus sequence variants present in infected plants and imperfectness of current diagnostic methods are illustrated and discussed. A failure of some primers to react (efficiently) with atypical isolates/strains of Apple chlorotic leaf spot virus (ACLSV) and Plum pox virus (PPV) is demonstrated. On the other hand, problematic positive reactions of PPV isolate L5 classified to D-type, but showing some atypical reaction, are presented and analysed briefly. Some recommendations how to address these problems in the field of plant viruses diagnostics are given.
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